Associate Research Scientist
Zizhang Sheng is an associated research scientist in the Honig-Shapiro labs. By taking the advantage of next-generation sequencing technique, he uses bioinformatics approaches to investigate features of B cell repertoires, and to study mechanisms modulating the maturation process of broadly HIV-1 neutralizing antibody lineages in humans and animal models, with the aim of finding an optimal HIV-1 vaccine design strategy. He also studies features and maturation process of auto-reactive B cells involved in autoimmune diseases. By using structural bioinformatics approaches, he is also studying functions of antibody somatic hypermutation and antibody affinity improvement.
Zizhang Sheng earned his PhD at Kunming Institute of Zoology, Chinese Academy of Science. His PhD research was focused on functional evolution of protein domain superfamilies.
PhD, Kunming Institute of Zoology, Chinese Academy of Science Molecular Evolution
BS, Shandong Normal University, China Biotechnology
* equal author contributions
Chaim A. Schramm*, Zizhang Sheng*, Zhenhai Zhang*, John R. Mascola, Peter D. Kwong, and Lawrence Shapiro. SONAR: A high-throughput pipeline for detecting antibody ontogenies in longitudinal deep-sequencing data. Front. Immunol., 7-372, (2016)
Zizhang Sheng, Chaim Schramm, Mark Connors, Lynn Morris, John R. Mascola, Peter D. Kwong, and Lawrence Shapiro. Effects of Darwinian selection and mutability on rate of broadly neutralizing antibody evolution during HIV-1 infection. PLoS Comp Bio 12 (5), e1004940, (2016)
Mattia Bonsignori*, Tongqing Zhou*, Zizhang Sheng*, Lei Chen*, Feng Gao, M. Gordon Joyce, Gabriel Ozorowski, Gwo-Yu Chuang, Chaim A. Schramm, Kevin Wiehe, S. Munir Alam, Todd Bradley, Morgan Gladden, Kwan-Ki Hwang, Sheelah Iyengar, Amit Kumar, Xiaozhi Lu, Kan Luo, Michael Mangiapani, Robert J. Parks, Hongshuo Song, Priyamvada Acharya, Robert T. Bailer, Allen Cao, Aliaksandr Druz, Ivelin S. Georgiev, Young D. Kwon, Mark K. Louder, Xueling Wu, Baoshan Zhang, Anqi Zheng, Brenna J. Hill, Rui Kong, Cinque Soto, NISC Comparative Sequencing Program, James C. Mullikin, Daniel C. Douek, David C. Montefiori, Michael A. Moody, George M. Shaw, Beatrice H. Hahn, Garnett Kelsoe, Peter T. Hraber, Bette T. Korber, Scott D. Boyd, Andrew Z. Fire, Thomas B. Kepler, Lawrence Shapiro, Andrew B. Ward, John R. Mascola, Hua-Xin Liao, Peter D. Kwong and Barton F. Haynes. Maturation Pathway from Germline to Broad HIV-1 Neutralizer of a CD4-Mimicking Antibody. Cell 165(2):449-63. (2016).
Xueling Wu*, Zhenhai Zhang*, Chaim A. Schramm*, M. Gordon Joyce*, Young Do Kwon*, Tongqing Zhou*, Zizhang Sheng*, Baoshan Zhang, Sijy O’Dell, Krisha McKee, Ivelin S. Georgiev, Gwo-Yu Chuang, Nancy S. Longo, Rebecca M. Lynch, Kevin O. Saunders, Cinque Soto, Sanjay Srivatsan, Yongping Yang, Robert T. Bailer, Mark K. Louder, NISC Comparative Sequencing Program, James C. Mullikin, Mark Connors, Peter D. Kwong, John R. Mascola, and Lawrence Shapiro. Maturation and diversity of the VRC01-antibody lineage over 15 years of chronic HIV-1 infection. Cell, 161(3), 470-85. (2015).
Francica JR, Sheng Z, Zhang Z, Nishimura Y, Shingai M, Ramesh A, Keele BF, Schmidt SD, Flynn BJ, Darko S, Lynch RM, Yamamoto T, Matus-Nicodemos R, Wolinsky D; NISC Comparative Sequencing Program, Nason M, Valiante NM, Malyala P, De Gregorio E, Barnett SW, Singh M, O'Hagan DT, Koup RA, Mascola JR, Martin MA, Kepler TB, Douek DC, Shapiro L, Seder RA. Analysis of immunoglobulin transcripts and hypermutation following SHIVAD8 infection and protein-plus-adjuvant immunization. Nat Commun. 2015 Apr 10;6:6565.
Wu X, Zhang Z, Schramm CA, Joyce MG, Do Kwon Y, Zhou T, Sheng Z, Zhang B, O'Dell S, McKee K, Georgiev IS, Chuang GY, Longo NS, Lynch RM, Saunders KO, Soto C, Srivatsan S, Yang Y, Bailer RT, Louder MK; NISC Comparative Sequencing Program, Mullikin JC, Connors M, Kwong PD, Mascola JR, Shapiro L. Maturation and diversity of the VRC01-antibody lineage over 15 years of chronic HIV-1 infection. Cell. 2015 Apr 8. pii: S0092-8674(15)00257-3.
Sundling C, Zhang Z, Phad GE, Sheng Z, Wang Y, Mascola JR, Li Y, Wyatt RT, Shapiro L, Karlsson Hedestam GB. Single-cell and deep sequencing of IgG-switched macaque B cells reveal a diverse Ig repertoire following immunization. J Immunol. 2014 Apr 15;192(8):3637-44.
Sheng Z*, Ran Z*, Wang D, Hoppe AD, Simonson R, Chakravarty S, Hause BM, Li F. Genomic and evolutionary characterization of a novel influenza-C-like virus from swine. Arch Virol. 2014 Feb;159(2):249-55.