Monitoring Translation in Space and Time with Ribosome Profiling

Jonathan Weissman
University of California, San Francisco / HHMI

Irving Cancer Research Center
1130 St. Nicholas Avenue
Room 816
New York, NY 10032

The ability to sequence genomes has far outstripped approaches for deciphering the information they encode. We have developed a suite of techniques based on ribosome profiling (deep sequencing of ribosome protected fragments) that dramatically expand our ability to follow translation in vivo. I will present recent applications of our ribosome profiling approach including the following: (1) Development of ribosome profiling protocols for a wide variety of eukaryotic and prokaryotic organisms. (2) Uses of ribosome profiling to globally monitor when chaperones, targeting factors or processing enzymes engage nascent chains. (3) Deciphering the driving force and biological consequences underlying the choice of synonymous codons. 

Event Series Name
Department of Systems Biology Seminar Series
Andrea Califano

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09-16-15 15:00:00
09-16-15 15:00:00
Jonathan Weissman (University of California, San Francisco / HHMI)
Monitoring Translation in Space and Time with Ribosome Profiling
Department of Systems Biology Common Room, ICRC 816