Detailed Map Gives Scientists a New Window into how Human-Infecting Viruses Work
Researchers implement P-HIPSTer, an in silico computational framework that leverages protein structure information to identify approximately 282,000 protein-protein interactions across all fully-sequenced human-infecting viruses (1001 in all). This image highlights that in addition to rediscovering known biology, P-HIPSTer has yielded a series of new findings and enables discovery of a previously unappreciated universe of cellular circuits and biological principles that act on human-infecting viruses. (Image Courtesy of Dr. Sagi Shapira)
Researchers at Columbia University Irving Medical Center have leveraged a computational method to map protein-protein interactions between all known human-infecting viruses and the cells they infect. The method, along with the data that it generated, has spawned a wealth of information toward improving our understanding of how viruses manipulate the cells that they infect and cause disease. Among its findings, the work uncovered a role for estrogen receptor in regulating Zika Virus (ZIKV) infection, as well as links between cancer and the human papillomavirus (HPV).
The research, led by Sagi Shapira , PhD, Assistant Professor in the Department of Systems Biology and the Department of Microbiology & Immunology at Columbia University Vagelos College of Physicians and Surgeons , appears today in the journal, Cell . Dr. Shapira’s collaborators include Professors Barry Honig , PhD, of Systems Biology and of Biochemistry and Molecular Biophysics and Raul Rabadan , PhD, of Systems Biology and of Biomedical Informatics.
From seasonal influenza and chicken pox, which are largely treatable, to life-threatening emerging viruses, such as Ebola and Zika, infections can result in a wide range of clinical symptoms and outcomes. At the molecular level, viruses invade cells and manipulate them to replicate, survive, and cause disease. Since they depend on human cells for their life cycle, one way viruses co-opt cellular machinery is through protein-protein interactions within their cell host. Similarly, cells respond to infection by initiating immune responses that control and limit viral replication – these too, depend on protein-protein interactions.
"There are over 1,000 unique viruses that are known to infect people, yet, despite their unquestionable public health importance, we know virtually nothing about the vast majority of them."
To date, considerable effort has been invested in identifying these key interactions – and many of these efforts have resulted in many fundamental discoveries, some with therapeutic implications. However, limitations on scalability, efficiency, and even access have limited the use of traditional methods. To address this challenge, Dr. Shapira and his collaborators developed and implemented a computational framework, termed P-HIPSTER , that infers interactions between pathogen and human proteins, the building blocks of viruses and cells.
Until now, our knowledge about many viruses that infect people is limited to their genome sequences. Yet, for most viruses little has been uncovered about the underlying biological interactions that drive these relationships and give rise to disease.
“There are over 1,000 unique viruses that are known to infect people,” says Dr. Shapira. “Yet, despite their unquestionable public health importance, we know virtually nothing about the vast majority of them. We just know they infect human cells. The idea behind this effort was to systematically catalogue the interactions that viruses have with the cells they infect. And, by doing so, also reveal some really interesting biology and provide the scientific community with a resource that they can use to make interesting observations of their own.”
Based on the PrePPI algorithm (developed in Professor Honig’s Laboratory), P-HIPSTer exploits protein structural information to systematically interrogate virus-human protein-protein interactions with remarkable accuracy. Dr. Shapira and his collaborators applied P-HIPSTer to all 1,001 human-infecting viruses and the approximately 13,000 proteins they encode. The algorithm predicted roughly 280,000 likely pairs of interacting proteins that represent a comprehensive catalogue of human virus protein-protein interactions with an accuracy rate of almost 80 percent.
“This is the first step towards building a comprehensive cartography of physical interactions between different organisms,” Dr. Shapira says.
Series of New Findings: Zika, HPV, Viral Evolution
In addition to defining pan-viral protein interactions, P-HIPSTer has yielded new biological insights into Zika virus, HPV, and the impact of viruses in shaping human genetics.
Among their discoveries, the researchers found that Zika virus interacts with estrogen receptor, the protein that allows cells to effectively respond to the estrogen hormone. Importantly, they found estrogen receptor has potential to inhibit Zika virus replication. Says Dr. Shapira, “And, in fact, estrogen receptor inhibits viral replication even more than interferon, a protein that is the body’s first line of defense to viral infection and our gold standard for anti-viral defense.”
The finding is particularly relevant to clinical disease as pregnant women are most susceptible to Zika during their first trimester, which is when estrogen levels are at their lowest. This period also is when the fetus is most susceptible to Zika, a virus for which there is no vaccine or specific treatment and that can cause severe birth defects.
Dr. Shapira and his team also explored interactions between human papillomavirus (HPV; the leading cause of cervical cancer) and the cells that it infects. HPV is the most common sexually transmitted infection with approximately 80 percent of sexually active individuals contracting one of the 200 different types of HPV at some point in their lives. Dr. Shapira and his team used the data generated by P-HIPSTer to identify protein-protein interactions that distinguish HPV infections associated with cancer from those that are not. In addition to providing insights into how HPV may cause disease, the finding could lead to improved diagnostics for those infected with HPV, and P-HIPSTer could potentially be used to help predict whether or not any particular virus is likely to be highly pathogenic.
The researchers also examined whether the interactions mediated by viruses have impacted human genetics. The researchers found evidence of strong selection pressure for several dozen cellular proteins have been shaped by viral infection, unlocking new insights into how our genome has been impacted by viruses.
“One of the things we can do with this data is drill down and ask whether virus infection has changed the history of human genetics,” notes Dr. Shapira. “That is certainly not a novel idea but to have a catalogue of what those proteins are is significant. There are a lot of areas that we can explore now that we couldn’t before.”
Dr. Shapira and his team intend to apply P-HIPSTer on more complex pathogens, such as parasites and bacteria, and use it to better understand how bacteria in the human gut communicate with each other. In the future, the algorithm could also be used to explore viruses or pathogens that effect agricultural plants or livestock.
The Shapira Laboratory at Columbia University is working to decipher the genetic and molecular circuitry at the interface of host–pathogen interactions. A deeper understanding of these relationships provides important insights into cellular machinery that control basic cell biology and has broad implications in human translational immunology and infectious disease research.
The paper, “ A Structure-Informed Atlas of Human-Virus Interactions ”, is also coauthored by: Gorka Lasso (Columbia Systems Biology and Microbiology & Immunology); Sandra V. Mayer (Columbia Systems Biology and Microbiology & Immunology); Evandro R. Winkelmann (Columbia Systems Biology and Microbiology & Immunology); Tim Chu (Columbia Systems Biology); Oliver Elliot (Columbia System Biology); Juan Angel Patino-Galindo (Columbia Systems Biology); and Kernyu Park (Columbia Biomedical Informatics).
Interactions derived by P-HIPSTer can be browsed at http://phipster.org . The study’s results are available through an interactive webserver that enables both searchable queries and data download.